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This Concept Map, created with IHMC CmapTools, has information related to: LAB 2 - ASEPTIC TECHNIQUE AND TRANSFER OF MICROORGANISMS - ASEPTIC TECHNIQUE, Aseptic Technique removing the inoculum from a broth culture, Very briefly flame the lip of the culture tube. step 3 Place the loopfull of bacteria into your sterile broth tube., Sterilize the inoculating loop by passing it at an angle through the flame of a gas burner until the entire length of the wire becomes orange from the heat. step 2 Lift the lid of the culture plate slightly and stab the loop into the agar away from any growth to cool the loop., Remove the cap of the pure culture tube with the little finger of your loop hand. Never lay the cap down or it may become contaminated. step 3 Very briefly flame the lip of the culture tube., 3 sector method incubate isolated colonies, Replace the cap. step 6 Sterilize the inoculating loop by passing it at an angle through the flame of a gas burner until the entire length of the wire becomes orange from the heat., Place the loopfull of bacteria into your sterile broth tube. step 4 Again flame the lip of the culture tube., Streak the loop across the surface of the agar medium using either the 3 sector or the 5 sector method. In order to avoid digging into the agar as you streak the loop over the top of the agar you must keep the loop parallel to the the agar surface. Always start streaking at the "12:00 position" of the plate and streak side-to-side as you pull the loop toward you. method 5 sector method, from a petri plate culture step 1 Sterilize the inoculating loop by passing it at an angle through the flame of a gas burner until the entire length of the wire becomes orange from the heat., inoculating a broth tube step 1 Remove the cap of the uninoculated culture tube with the little finger of your loop hand. Never lay the cap down or it may become contaminated., Remove the cap of the uninoculated culture tube with the little finger of your loop hand. Never lay the cap down or it may become contaminated. step 2 Very briefly flame the lip of the culture tube., inoculating a petri plate step 1 If the agar surface of the plate is visibly wet, use a sterile swab to gently remove the water., Lift the lid of the culture plate slightly and stab the loop into the agar away from any growth to cool the loop. step 3 Scrape off a small amount of the organisms and close the lid., 5 sector method step 4 Sterilize the inoculating loop by passing it at an angle through the flame of a gas burner until the entire length of the wire becomes orange from the heat., Streak the loop across the surface of the agar medium using either the 3 sector or the 5 sector method. In order to avoid digging into the agar as you streak the loop over the top of the agar you must keep the loop parallel to the the agar surface. Always start streaking at the "12:00 position" of the plate and streak side-to-side as you pull the loop toward you. method 3 sector method, Aseptic Technique removing the inoculum from a petri plate culture, Insert the sterile inoculating loop and remove a loopful of the inoculum. step 5 Again flame the lip of the culture tube., Lift the edge of the lid just enough to insert the loop. step 3 Streak the loop across the surface of the agar medium using either the 3 sector or the 5 sector method. In order to avoid digging into the agar as you streak the loop over the top of the agar you must keep the loop parallel to the the agar surface. Always start streaking at the "12:00 position" of the plate and streak side-to-side as you pull the loop toward you., 3 sector method step 4 Sterilize the inoculating loop by passing it at an angle through the flame of a gas burner until the entire length of the wire becomes orange from the heat., Aseptic Technique inoculating a sterile medium inoculating a petri plate
